Research Articles
Beyond a Simple Cleavage: The 89 kDa and 24 kDa PARP-1 Fragments as Critical Switches in Cell Fate and Disease
This article provides a comprehensive analysis of the 89 kDa and 24 kDa fragments generated by the caspase-mediated cleavage of PARP-1, a key event in cellular stress response.
Caspase-3 and Caspase-7 in PARP-1 Cleavage: Molecular Mechanisms, Functional Divergence, and Therapeutic Implications
This article provides a comprehensive analysis of the distinct yet complementary roles of caspase-3 and caspase-7 in poly(ADP-ribose) polymerase-1 (PARP-1) cleavage, a critical event governing cell fate decisions between apoptosis,...
PARP-1 Cleavage in Apoptosis: From a Molecular Switch to Diagnostic and Therapeutic Opportunities
This article provides a comprehensive analysis of the critical functional differences between full-length and cleaved PARP-1 during apoptosis, a key event in cellular fate.
PARP-1 Cleavage: The Definitive Apoptosis Marker and Its Emerging Roles in Cell Death
This article provides a comprehensive analysis of poly(ADP-ribose) polymerase-1 (PARP-1) cleavage as a fundamental biomarker of apoptosis.
Tissue-Specific Caspase Expression: Validation Methods, Biological Significance, and Clinical Implications
This comprehensive review explores the critical importance of validating tissue-specific caspase expression patterns across normal physiology and disease states.
Intrinsic vs. Extrinsic Apoptosis: A Comparative Analysis of Initiation Mechanisms and Therapeutic Applications
This article provides a comprehensive comparative analysis of the intrinsic (mitochondrial) and extrinsic (death receptor) pathways of apoptotic initiation.
Caspase-3: The Double-Edged Sword in Cell Fate - From Apoptotic Executioner to Non-Apoptotic Regulator
This article provides a comprehensive synthesis of the dualistic roles of caspase-3, a cysteine-aspartic acid protease historically recognized as the primary executioner of apoptosis.
DEVDase vs. PARP Cleavage: A Comprehensive Guide to Cross-Validating Caspase Activity in Apoptosis Research
This article provides researchers, scientists, and drug development professionals with a detailed framework for cross-validating caspase activity measurements by comparing the widely used DEVDase assay with PARP cleavage analysis.
tBid Generation: Comparing Caspase-8 and Caspase-2 Efficiency in Apoptotic Signaling
This article provides a comprehensive analysis of the mechanisms and relative efficiencies of caspase-8 and caspase-2 in generating truncated Bid (tBid), a pivotal event in the mitochondrial apoptotic pathway.
Caspase Activation vs. Annexin V vs. TUNEL: A Researcher's Guide to Apoptosis Marker Selection
This article provides a comprehensive comparison of three cornerstone apoptosis detection methods—caspase activation, Annexin V binding, and TUNEL assay.