Research Articles
Beyond Apoptosis: Validating Caspase-3 Knockout in Cell Migration and Invasion Assays
This article provides a comprehensive resource for researchers and drug development professionals on the critical role of caspase-3 in non-apoptotic cellular processes, specifically cancer cell migration and invasion.
Induced Conformation vs. Induced Proximity: Unraveling the Molecular Mechanisms of Caspase-9 Activation
The activation of caspase-9, the initiator caspase of the intrinsic apoptosis pathway, is a pivotal event in programmed cell death with profound implications for cancer therapy and degenerative diseases.
Pan-Caspase vs. Specific Caspase Inhibitors: A Comparative Analysis of Efficacy, Applications, and Clinical Translation
Caspase inhibitors represent a promising therapeutic strategy for pathologies involving dysregulated cell death, such as inflammatory, neurodegenerative, and metabolic diseases, as well as ischemia-reperfusion injury.
Decoding Executioner Caspases: A Comprehensive Guide to Caspase-3 and Caspase-7 Substrate Specificity
This article provides a detailed analysis of the substrate specificity profiles of the key executioner caspases, caspase-3 and caspase-7.
Troubleshooting Nonspecific Cleavage in Caspase Substrates: A Researcher's Guide to Enhancing Specificity and Data Reliability
This article provides a comprehensive guide for researchers, scientists, and drug development professionals facing the common yet challenging issue of nonspecific cleavage when working with caspase substrates.
Optimizing Caspase Inhibitor Concentration and Timing: A Strategic Guide for Preclinical Research
This article provides a comprehensive guide for researchers and drug development professionals on optimizing the concentration and timing of caspase inhibitors in experimental models.
Decoding Caspase Activation: A Strategic Guide to Distinguish Apoptosis from Pyroptosis in Research and Drug Discovery
This article provides a comprehensive guide for researchers and drug development professionals on distinguishing between apoptotic and pyroptotic caspase activation.
Strategies for Preventing Photobleaching in Long-Term Caspase Imaging: A Guide for Biomedical Researchers
Long-term live-cell imaging of caspase activity is crucial for studying apoptosis in drug development and disease research, but it is severely hampered by photobleaching.
Optimizing DEVD Caspase-3/7 Assays: A Guide to Enhancing Specificity and Accuracy in Apoptosis Research
This article provides a comprehensive guide for researchers and drug development professionals on optimizing the specificity of DEVD-based assays for caspase-3 and caspase-7 activity.
Heterogeneous Caspase Activation in Organoid Models: From Mechanisms to Applications in Drug Discovery
This article explores the critical role of heterogeneous caspase activation within three-dimensional organoid models, a key phenomenon in understanding variable cellular responses to therapy.