Research Articles
A Comprehensive Protocol for Inducing and Analyzing Apoptosis in HL-60 Cell Cultures
This article provides a detailed, step-by-step guide for researchers and drug development professionals on inducing and quantifying apoptosis in the human promyelocytic HL-60 cell line.
Automated Algorithms for Apoptotic Biomarker Translocation Analysis: Advancing High-Throughput Drug Discovery
This article provides a comprehensive resource for researchers and drug development professionals on the implementation of automated algorithms for analyzing dynamic apoptotic event translocation.
Constructing Cytochrome C-GFP Reporter Cell Lines: A Comprehensive Guide from Design to High-Throughput Application
This article provides a complete roadmap for researchers and drug development professionals on the construction and application of cytochrome C-green fluorescent protein (GFP) reporter cell lines.
Cleaved Caspase-3 Immunohistochemistry: A Comprehensive Guide from Detection to Clinical Application
This article provides a comprehensive resource for researchers and drug development professionals on cleaved caspase-3 immunohistochemistry (IHC), a critical technique for detecting apoptotic cells in tissue specimens.
Detecting Apoptosis: A Comprehensive Guide to PARP-1 Cleavage Western Blot Assay
This article provides researchers, scientists, and drug development professionals with a complete guide to using Western blotting for detecting apoptosis through PARP-1 cleavage analysis.
Hoechst and DAPI in Fluorescence Microscopy: A Comprehensive Guide to Chromatin Condensation Analysis
This article provides a thorough exploration of Hoechst and DAPI fluorescent dyes as powerful tools for investigating chromatin condensation and nuclear architecture.
TMRE Mitochondrial Membrane Potential Analysis: A Comprehensive Guide for Biomedical Research and Drug Discovery
This article provides a comprehensive resource for researchers, scientists, and drug development professionals on the application of Tetramethylrhodamine Ethyl Ester (TMRE) for analyzing mitochondrial membrane potential (ΔΨm).
Annexin V Staining Flow Cytometry Protocol: A Complete Guide for Apoptosis Detection
This comprehensive guide provides researchers, scientists, and drug development professionals with a complete framework for planning, executing, and validating Annexin V staining experiments for apoptosis detection via flow cytometry.
Mastering the TUNEL Assay: A Complete Protocol and Troubleshooting Guide for Tissue Sections
This article provides a comprehensive guide to the Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, a cornerstone technique for detecting DNA fragmentation associated with cell death in tissue...
Apoptosis in MODS: Molecular Mechanisms, Biomarker Discovery, and Emerging Therapeutic Strategies
Multiple organ dysfunction syndrome (MODS) remains a leading cause of mortality in critically ill patients, with apoptosis occupying a central role in its pathogenesis.